This dataset contains profiles of temperature, dissolved oxygen, salinity, and other observations collected by Seagliders during the Sub-Mesoscale Ocean Dynamics Experiment (S-MODE) field campaign. The experiment was conducted approximately 300 km offshore of San Francisco, during two intensive operating periods in Fall 2022 and Spring 2023. S-MODE aims to understand how ocean dynamics acting on short spatial scales influence the vertical exchange of physical and biological variables in the ocean. Seagliders are autonomous underwater vehicles (AUVs) designed to glide from the ocean surface to as deep as 1000 m and back whilecollecting profiles of oceanic variables. Data are available in netCDF format.