The proteomics experiment involved analyzing the protein expression profiles of Enterobacter cloacae under different gravity conditions simulated in High Aspect Ratio Vessels (HARVs). The three conditions studied were normal gravity (NG), inverted normal gravity (INV), and low shear modeled microgravity (LSMMG). The goal was to assess how E. cloacae adapts to microgravity, given its relevance to astronaut health during spaceflight. By comparing the proteomic profiles across these conditions, the study identified significant changes in protein expression in LSMMG and INV compared to NG.